Nanosilicate embedded agarose hydrogels with improved bioactivity

Topuz F., Nadernezhad A., Caliskan O. S., Menceloglu Y. Z., Koc B.

CARBOHYDRATE POLYMERS, cilt.201, ss.105-112, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 201
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.carbpol.2018.08.032
  • Dergi Adı: CARBOHYDRATE POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.105-112
  • Anahtar Kelimeler: Agarose, Laponite, Bioactive hydrogels, Nanocomposite, Nanosilicates, INTERPENETRATING NETWORK HYDROGELS, CELL-ADHESION, IN-VITRO, GELS, NANOCOMPOSITES, AGGRECAN, GELATION
  • İstanbul Teknik Üniversitesi Adresli: Hayır

Özet

This paper reports the synthesis of nanocomposite agarose hydrogels with improved bioactivity with the incorporation of anisotropic 2D nanosilicates (Laponite) to promote cell binding, growth and proliferation. Rheological measurements showed that the incorporation of nanosilicates slightly increased the gelation temperature (T-gel). The use of higher nanosilicate content at the constant agarose concentration improved the mechanical properties of the gels. Due to the non-swelling nature of agarose, the addition of nanosilicates did not result in any remarkable change in the swelling properties of the agarose gels, while collapsed agarose nanofibers were observed with the incorporation of nanosilicates. EDX analysis confirmed the presence of the embedded nanosilicates in the gel matrix. The existence of physical interactions between nanosilicate and agarose was demonstrated by FTIR over the shifting of Si-O stretching band to a lower frequency. The encapsulated NIH/3T3 fibroblast cells showed enhanced proliferation and spreading in the presence of nanosilicates.