Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cifuentes A., Poppe H., Kraak J. C., Erim F. B.

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, cilt.681, sa.1, ss.21-27, 1996 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 681 Sayı: 1
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1016/0378-4347(96)00004-7
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.21-27
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

A study of the capillary electrophoretic separations of proteins and peptides using high-molecular-mass polyethyleneimine (PEI) is presented. Experiments were performed in the PEI-coated capillaries together with the use of this polymer as a buffer additive under different separation conditions. The effects of pH and the concentration of PEI in the buffer on the electroosmotic flow and the migration orders of biopolymers were investigated. The use of the cationic polymer offers an alternative for the modification of the separation selectivity and resolution of biopolymers.