Simultaneous Determination of Nitrate and Nitrite in Fish Products with Improved Sensitivity by Sample Stacking-Capillary Electrophoresis

Kalaycıoğlu Z., Erim F. B.

FOOD ANALYTICAL METHODS, cilt.9, sa.3, ss.706-711, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s12161-015-0241-4
  • Dergi Adı: FOOD ANALYTICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.706-711
  • Anahtar Kelimeler: Capillary electrophoresis, Inorganic anions, Sample stacking, Nitrite, Nitrate, Fish, BIOGENIC-AMINES, ZONE-ELECTROPHORESIS, SALTED FISH, VEGETABLES, MEAT, TURKEY
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

A fast and reproducible method for the simultaneous determination of nitrate and nitrite ions in canned fish samples by capillary zone electrophoresis has been developed. The sensitivity of the method was increased by applying a sample stacking technique. Optimal separation conditions were selected as 30 mmol L-1 formic acid and 30 mmol L-1 sodium sulfate at a pH of 4.0. The separation of nitrate and nitrite ions was achieved within 2.5 min. The limits of detection obtained at a signal-to-noise ratio of 3 for nitrate and nitrite were 0.55 and 0.82 mu mol L-1, while the relative standard deviations of intra-day corrected peak areas were 0.99 and 2.74 %, respectively. Recovery values ranged between 88.7 and 104 % for both ions. The method was successfully applied to canned fish samples, namely tuna, mackerel and sardine.