Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

Pala Özkök İ., Ubay-Cokgor E., Jonas D., Orhon D.

JOURNAL OF HAZARDOUS MATERIALS, cilt.367, ss.418-426, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 367
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.jhazmat.2018.12.094
  • Dergi Adı: JOURNAL OF HAZARDOUS MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.418-426
  • Anahtar Kelimeler: Aerobic processes, Tetracycline, Chronic inhibition, Activated sludge modeling, High-throughput sequencing, WASTE-WATER, GENE CASSETTES, ANTIBIOTICS, BIODEGRADATION, REPRESSOR, ERYTHROMYCIN, COMPLEX, GROWTH
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.