Microfluidic cell sorter with integrated piezoelectric actuator


Chen C. H., CHO S. H., TSAİ F., Erten A. C., LO Y.

BIOMEDICAL MICRODEVICES, cilt.11, sa.6, ss.1223-1231, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s10544-009-9341-5
  • Dergi Adı: BIOMEDICAL MICRODEVICES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1223-1231
  • Anahtar Kelimeler: Hydrodynamics, Flow cytometer, Microfluidics, Sorter, Piezoelectric actuation, SINGLE MAMMALIAN-CELLS, ON-CHIP, DIELECTROPHORESIS, SEPARATION, PARTICLES, DEVICES
  • İstanbul Teknik Üniversitesi Adresli: Hayır

Özet

We demonstrate a low-power (< 0.1 mW), low-voltage (< 10 Vp-p) on-chip piezoelectrically actuated micro-sorter that can deflect single particles and cells at high-speed. With rhodamine in the stream, switching of flow between channels can be visualized at high actuation frequency (similar to 1.7 kHz). The magnitude of the cell deflection can be precisely controlled by the magnitude and waveform of input voltage. Both simulation and experimental results indicate that the drag force imposed on the suspended particle/cell by the instantaneous fluid displacement can alter the trajectory of the particle/cell of any size, shape, and density of interest in a controlled manner. The open-loop E. Coli cell deflection experiment demonstrates that the sorting mechanism can produce a throughput of at least 330 cells/s, with a promise of a significantly higher throughput for an optimized design. To achieve close-loop sorting operation, fluorescence detection, real-time signal processing, and field-programmable-gate-array (FPGA) implementation of the control algorithms were developed to perform automated sorting of fluorescent beads. The preliminary results show error-free sorting at a sorting efficiency of similar to 70%. Since the piezoelectric actuator has an intrinsic response time of 0.1-1 ms and the sorting can be performed under high flowrate (particle speed of similar to 1-10 cm/s), the system can achieve a throughput of > 1,000 particles/s with high purity.