Enzymatic esterification of (-)-menthol with lauric acid in isooctane by sorbitan monostearate-coated lipase from Candida rugosa


Babali B., AKSOY H., Tuter M., USTUN G.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, vol.78, no.2, pp.173-175, 2001 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 2
  • Publication Date: 2001
  • Doi Number: 10.1007/s11746-001-0239-6
  • Journal Name: JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.173-175
  • Keywords: Candida rugosa lipase, esterification, lauric acid, menthol, sorbitan monostearate, MENTHOL, SELECTIVITY

Abstract

Esterification of (-)-menthol and (+/-)-menthol with lauric acid in isooctane was successfully catalyzed by a commercial nonionic surfactant (sorbitan monostearate)-coated lipase from Candida rugosa (Lipase AY "Amano" 30) at the molar ratio of 1:1 and at 35 degreesC using 1.5 g enzyme/g (-)-menthol and 0.1-g molecular sieves. After 1 h, molar conversion of (-)-menthol reached 81%. Equilibrium was reached after ca. 4 h, giving a (-)-menthol molar conversion of 94%. Under the same conditions, native lipase catalyzed the esterification of (-)-menthol and lauric acid to yield a molar conversion,of 93% after 72 h. Coating the lipase with sorbitan monostearate increased the esterification rates of both (-)-menthol and (+/-)-menthol with lauric acid. After 6 h, the molar conversions of (-)-menthol and (+/-)menthol were 94, and 62%, respectively.