Indian Journal Of Chemistry Section A-Inorganic Bio-Inorganic Physical Theoretical & Analytical Chemistry, vol.59, no.3, pp.317-323, 2020 (SCI-Expanded)
A facile, environmentally friendly one-step reaction for the preparation of luminescent N-doped graphene quantum dots
(GQDs) involving a hydrothermal reaction between citric acid and 2-aminoethylphosphonic acid has been designed.
Graphene quantum dots have been characterized by UV-visible absorption, FTIR spectroscopy, transmission electron
microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS) techniques.
Furthermore, the biological activity of the GQDs has been studied. UV-visible spectroscopy studies of the interactions
between the GQDs and calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via intercalative
binding. DNA cleavage study showed that the GQDs cleaved DNA oxidatively. In addition, antioxidant activity of N-doped
GQDs was measured using the DPPH method. As the concentration of the compound increased, the antioxidant activity also
has increased. According to cell viability analyses results, the N-doped GQDs showed cell viability (70%) when the concentration
reaches 228 μg/mL for A549, 200 μg/mL for MDA-MB-231 and 140 μg/mL for NIH-3T3 cell lines with 24 h incubation.
Keywords: N-doped graphene quantum dots, Biological activity, Antioxidant activity, MTT, Cell viability