Disturbed excitation energy transfer in Arabidopsis thaliana mutants lacking minor antenna complexes of photosystem II


Osto L., Ünlü C. , Cazzaniga S., van Amerongen H.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, vol.1837, no.12, pp.1981-1988, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1837 Issue: 12
  • Publication Date: 2014
  • Doi Number: 10.1016/j.bbabio.2014.09.011
  • Title of Journal : BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
  • Page Numbers: pp.1981-1988
  • Keywords: Photosynthesis, Light-harvesting complex, Photosystem II, Time-resolved fluorescence spectroscopy, Thylakoid membrane, LIGHT-HARVESTING-COMPLEX, PRIMARY CHARGE SEPARATION, CHLAMYDOMONAS-REINHARDTII, CRYSTAL-STRUCTURE, REDOX STATE, WILD-TYPE, FLUORESCENCE, PROTEINS, PLANT, ORGANIZATION

Abstract

Minor light-harvesting complexes (Lhcs) CP24, CP26 and CP29 occupy a position in photosystem II (PSII) of plants between the major light-harvesting complexes LHCII and the PSII core subunits. Lack of minor Lhcs in vivo causes impairment of PSII organization, and negatively affects electron transport rates and photoprotection capacity. Here we used picosecond-fluorescence spectroscopy to study excitation-energy transfer (EET) in thylakoid membranes isolated from Arabidopsis thaliana wild-type plants and knockout lines depleted of either two (koCP26/24 and koCP29/24) or all minor Lhcs (NoM). In the absence of all minor Lhcs, the functional connection of LHCII to the PSII cores appears to be seriously impaired whereas the "disconnected" LHCII is substantially quenched. For both double knock-out mutants, excitation trapping in PSII is faster than in NoM thylakoids but slower than in WT thylakoids. In NoM thylakoids, the loss of all minor Lhcs is accompanied by an over-accumulation of LHCII, suggesting a compensating response to the reduced trapping efficiency in limiting light, which leads to a photosynthetic phenotype resembling that of low-light-acclimated plants. Finally, fluorescence kinetics and biochemical results show that the missing minor complexes are not replaced by other Lhcs, implying that they are unique among the antenna subunits and crucial for the functioning and macro-organization of PSII. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license