We studied the effect of geometry on single focusing of particles in the passive microfluidic channels. There is a quantitative analysis of focusing on non-axisymmetric straight channels with two different unique designs as well as curved channels having symmetrical and asymmetrical radius of curvatures in one turn. We found that there is a clear relationship in existence of single line focusing with the degree of non-symmetry in microchannels. One-degree of asymmetry in straight channels does not induce any formation of single focusing, but single line focusing is pronounced by two-degree of asymmetry in straight channels. On the other hand, single line focusing in the curved channels is enhanced with asymmetrical radius of curvatures in one turn. The single line focusing in curved channels is seen at Rep values higher than 1, which gives us better continuous and high-throughput performance. The position of single focused particles in the microchannels is found to be 50 mu m with respect to the channel wall in the asymmetrical curved microchannels.