Many research studies have shown that bridges are vulnerable to earthquakes, graphically confirmed by incidents such as the San Fernando (1971 USA), Northridge (1994 USA), Great Hanshin (1995 Japan), and Chi-Chi (1999 Taiwan) earthquakes, amongst many others. The studies show that fragility curves are useful tools for bridge seismic risk assessments, which can be generated empirically or analytically. Empirical fragility curves can be generated where damage reports from past earthquakes are available, but otherwise, analytical fragility curves can be generated from structural seismic response analysis. Earthquake damage data in Turkey are very limited, hence this study employed an analytical method to generate fragility curves for the Alasehir bridge. The Alasehir bridge is part of the Manisa-Usak-Dumlupmar-Afyon railway line, which is very important for human and freight transportation, and since most of the country is seismically active, it is essential to assess the bridge's vulnerability. The bridge consists of six 30 m truss spans with a total span 189 m supported by 2 abutments and 5 truss piers, 12.5, 19, 26, 33, and 40m. Sap2000 software was used to model the Alasehir bridge, which was refined using field measurements, and the effect of 60 selected real earthquake data analyzed using the refined model, considering material and geometry nonlinearity. Thus, the seismic behavior of Alasehir railway bridge was determined and truss pier reaction and displacements were used to determine its seismic performance. Different intensity measures were compared for efficiency, practicality, and sufficiency and their component and system fragility curves derived.