The effects of hanger replacement from inclined to vertical configuration on seismic response of long-span suspension bridges are investigated considering multi-support earthquake excitation. The Bosphorus Bridge is investigated due to its recent comprehensive rehabilitation, mainly involving hanger replacement. The finite-fault stochastic simulation method (FINSIM) is utilized for multi-point earthquake time-history generation. The developed finite element (FE) model both for the inclined and vertical hanger arrangement are verified through the structural health monitoring (SHM) data. Based on the comparative analysis, the tension force of vertical hangers is found to be lower than that of inclined hangers, whereas the tension force of the main and back-stay cables remains the same. The compressive axial force of the deck decreases relatively in the case of the vertical hanger arrangement, whereas the cross-sectional forces at the tower base section increase. The approach viaducts are not affected by the vertical hanger arrangement. According to the demand/capacity ratios for damage estimation under the max. earthquake (2475 years return period), structural damage on the tower base section may be expected for both hanger arrangements, while these sections perform well under design scenario earthquake. The expansion joint of the bridge with inclined hangers is also estimated to be damaged; however, this displacement is lower in the case of the vertical hanger arrangement due to the viscous dampers. The findings also reveal that a change in hanger form of a suspension bridge can necessitate other structural retrofit, such as using viscous dampers to limit longitudinal displacements of the deck and retrofitting the bridge towers.