Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections

Akalın P., Karataş A.

MethodsX, cilt.11, 2023 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.mex.2023.102372
  • Dergi Adı: MethodsX
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, BIOSIS, Directory of Open Access Journals
  • Anahtar Kelimeler: COVID-19, Diagnostics, Influenza, Loop-Mediated Isothermal Amplification (LAMP), Nucleic acid lateral flow immunoassay (NALFIA), U-NALF
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Viral infections continue to pose a significant threat to the public health, leading to high morbidity and mortality rates worldwide. To combat these challenges, early detection and treatment are essential in reducing hospitalizations and preventing severe complications. Simple, inexpensive, and sensitive diagnostic methods are in constant demand in many areas. In this study, we report the development of a nucleic acid-based lateral flow immunoassay device (NALFIA) and demonstrate its successful application in conjunction with a multiplexed reverse-transcription loop-mediated isothermal amplification assay (LAMP) for the detection of SARS-CoV-2 and influenza. In our approach the NALFIA part preparation is independent of the target, and has the potential to ensure widespread use in diagnostics particularly where testing speed is critical such as in respiratory viral infections. • Simple, inexpensive, sensitive and reliable rapid diagnostic tool. • Target independent design. • Effective use for respiratory samples due to practical sample extraction.