Metro lines, which have become one of the most important transportation routes for today's cities, require long design and implementation processes. Their design load is mostly due to detailed engineering calculations, have to expand to meet the needs of the ever-increasing population. The behavior of slime moulds to find the shortest path they use to reach the food source has inspired models created specifically to test the accuracy of highway routes. The hypothesis of this study is that the swarm behavior of slime moulds and the routes they follow may produce the same or similar results as the existing metro lines. In this research, an answer was sought to the question of whether a metro line designed with an approach based on swarm intelligence could be compatible with the existing line.