Design of Colloidally Stable and Non-Toxic Petox-Based Polymersomes for Cargo Molecule Encapsulation

ÖZ U. C. , KÜÇÜKTÜRKMEN B., ÖZKÖSE U. U. , Gulyuz S., Bolat Z. B. , TELCİ D., ...More

CHEMNANOMAT, vol.5, no.6, pp.766-775, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1002/cnma.201800606
  • Title of Journal : CHEMNANOMAT
  • Page Numbers: pp.766-775


Polymersomes are vesicular constructs formed by the self-assembly of polymer amphiphiles. Here we report the fabrication of PEtOx-b-PLA polymersomes at physiological pH/salt concentration and the determination of a f(PEtOx) range, which is one of the most recognized factors that governs the self-assembly of amphiphilic copolymers, for the first time. The findings indicate that PEtOx-b-PLA copolymers, containing PEtOx fractions of varied lengths, self-assemble to form polymersomes in the range of 98.35 to 119.50 nm. The encapsulation potential of PEtOx-b-PLA polymersomes was demonstrated by loading of docetaxel (hydrophobic) with 17.90% encapsulation efficiency and by loading of pDNA (hydrophilic) with 65.03% encapsulation efficiency. In addition, size distribution profiles showed that polymersomes are colloidally stable for 24 h at 37 degrees C and for 3 months at +4 degrees C. The cell viability results showed that PEtOx-b-PLA copolymer and polymersomes are not cytotoxic to HUVEC, HEK293 and hMSC cell lines.