Effect of direct enzyme addition on the anaerobic digestion of OFMSW

Kabakci Y., Kosar S., Dogan O., Uctug F. G., Arıkan O. A.

Sustainable Chemistry and Pharmacy, vol.37, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 37
  • Publication Date: 2024
  • Doi Number: 10.1016/j.scp.2023.101415
  • Journal Name: Sustainable Chemistry and Pharmacy
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Alpha amylase, Anaerobic digestion, Biochemical methane potential (BMP), Direct dose, Enzyme treatment, Organic fraction of municipal solid waste (OFMSW)
  • Istanbul Technical University Affiliated: Yes


The organic fraction of municipal solid waste (OFMSW) has high biodegradability and is a good feedstock for anaerobic digestion (AD). However, the solubilization of OFMSW is a limiting factor for AD. In this study, enzyme treatment was investigated to increase the conversion of complex materials of OFMSW into soluble fractions. Six different concentrations (0.15, 0.3, 0.5, 1.0, 1.5 and 3.0 mg/VSadded) of the alpha amylase enzyme (obtained from Aspergillus oryzae) were directly added as a powder and automated biochemical methane potential (BMP) tests were performed under thermophilic conditions. According to BMP tests, the methane (CH4) yield increased up to 17.5 % and the lag phase decreased to 19.3 % after enzyme addition. The highest CH4 yield and shortest lag phase were obtained when 0.5 mg of enzyme/VSadded was added to the reactor. These findings suggest that direct alpha amylase enzyme application obtained from Aspergillus oryzae can be used to increase biogas production by enhancing solubilization of OFMSW for AD.