Assessment of the Wider Economic Impacts (WEIs) of transport infrastructures has become crucial for justifying transport infrastructure investments, especially large-scale projects, not only for transport researchers but also for policy makers. Although the WEIs have been widely discussed in transport research and policy for more than a decade, these discussions lacked an important dimension: the likelihood of transport infrastructure investments to produce spatio-economic inequalities. This paper discusses the necessity of incorporating an understanding of spatio-economic inequalities in the analysis of WEIs. In order to do that, an ex-post analysis of the Istanbul Metro is carried out. In this sense, this paper also adds to the growing number of ex-post analysis of transport infrastructures. Through its findings based on the ex-post analysis of the impacts of the Istanbul Metro, this paper concludes that contemplating spatio-economic inequalities in ex-ante analysis of transport infrastructure investments will improve the calculation of WEIs of transport infrastructures. Such an approach is expected to help policy makers investing in projects that are less likely to produce spatio-economic inequalities. (C) 2015 Elsevier Ltd. All rights reserved.