Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole


CIRPAN A., ALKAN S., TOPPARE L., Cianga I., Yagci Y.

DESIGNED MONOMERS AND POLYMERS, vol.6, no.3, pp.237-243, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2003
  • Doi Number: 10.1163/156855503768336252
  • Title of Journal : DESIGNED MONOMERS AND POLYMERS
  • Page Numbers: pp.237-243

Abstract

Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.