In the cooling of high heat capacity systems, immersion cooling systems are increasing importance due to the insufficient heat dissipation rates of conventional cooling systems. In this study, the effects of ultrasonic sound waves on cooling performance in single-phase immersion cooling systems were investigated. Experimental study was carried out with ultrasonic sound waves for 9.6 kHz, 14.4 kHz, 19.2 kHz, 24 kHz and silent conditions. In addition, the effects of discrete heat sources on thermal performance were investigated by changing the surface (embedded, 2 mm protruding, 4 mm protruding) and location configurations. According to the results obtained, the highest heat transfer coefficient was calculated as 1703.36 W/m2 °C for the embedded heat source closest to the tank bottom for at 24 kHz frequency. The lowest heat transfer coefficient was calculated as 373.28 W/m2 °C for the maximum protrusion amont heat source closest to the tank upper and when ultrasound manipulation was not applied. In addition, the immersion cooling heat transfer coefficient was increased by 107.49% with ultrasonic sound wave. The heat transfer coefficient increased with increasing ultrasound frequency and decreased with increasing protrusion thickness.