Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods

Ulker C., GOKALP N., Guvenilir Y.

BIOCATALYSIS AND BIOTRANSFORMATION, cilt.34, sa.4, ss.172-180, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 34 Konu: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/10242422.2016.1247818
  • Sayfa Sayıları: ss.172-180


In the present study, the recovery of activity of Candida antarctica lipase B (CALB) immobilized onto surface-modified rice husk ash (RHA) was 90% for both cross-linking and adsorption methods. Both cross-linked and adsorbed immobilized preparations were very stable, retaining more than 48% of their activity over the range of temperatures studied. The optimum temperature and optimum pH values were 37 degrees C and 7.0, respectively for both immobilized preparations, while the relative activities after storage at 4.0 degrees C for 60 days were 55% and 65% using cross-linking and adsorption methods, respectively. Also, the activity of the immobilized lipase began to decrease after 10 cycles, more than 58% of the initial activities were still retained after 10 cycles for both immobilization methods. These results indicated that lipase immobilized by cross-linking and adsorption not only effected activity recovery, but also remarkably effected stability, reusability and application adaptability. It can be concluded that, surface-modified RHA can be used as alternative supports for immobilization of CALB for polymerization reactions.