Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods


Ulker C. , GOKALP N., Guvenilir Y.

Biocatalysis and Biotransformation, vol.34, no.4, pp.172-180, 2016 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.1080/10242422.2016.1247818
  • Title of Journal : Biocatalysis and Biotransformation
  • Page Numbers: pp.172-180

Abstract

© 2016 Informa UK Limited, trading as Taylor & Francis Group.In the present study, the recovery of activity of Candida antarctica lipase B (CALB) immobilized onto surface-modified rice husk ash (RHA) was 90% for both cross-linking and adsorption methods. Both cross-linked and adsorbed immobilized preparations were very stable, retaining more than 48% of their activity over the range of temperatures studied. The optimum temperature and optimum pH values were 37 °C and 7.0, respectively for both immobilized preparations, while the relative activities after storage at 4.0 °C for 60 days were 55% and 65% using cross-linking and adsorption methods, respectively. Also, the activity of the immobilized lipase began to decrease after 10 cycles, more than 58% of the initial activities were still retained after 10 cycles for both immobilization methods. These results indicated that lipase immobilized by cross-linking and adsorption not only effected activity recovery, but also remarkably effected stability, reusability and application adaptability. It can be concluded that, surface-modified RHA can be used as alternative supports for immobilization of CALB for polymerization reactions.