Selective and Reversible Binding of Thiol-Functionalized Biomolecules on Polymers Prepared via Chemical Vapor Deposition Polymerization


Ross A., Durmaz H. , CHENG K., DENG X., Liu Y., Oh J., ...Daha Fazla

LANGMUIR, cilt.31, ss.5123-5129, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 31 Konu: 18
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/acs.langmuir.5b00654
  • Dergi Adı: LANGMUIR
  • Sayfa Sayıları: ss.5123-5129

Özet

We use chemical vapor deposition polymerization to prepare a novel dibromomaleimide-functionalized polymer for selective and reversible binding of thiol-containing biomolecules on a broad range of Substrates. We report the synthesis and CVD polymerization of 4-(3,4-dibromomaleimide)[2.2]paracyclophane to yield nanometer thick polymer coatings. Fourier transformed infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the chemical composition of the polymer coating. The reactivity of the polymer coating toward thiol-functionalized molecules was confirmed using fluorescent ligands. As a proof of concept, the binding and subsequent release of cysteine-modified peptides from the polymer coating were also demonstrated via sum frequency generation spectroscopy. This reactive polymer coating provides a flexible surface modification approach to selectively and reversibly bind biomolecules on a broad range of materials, which Could open up new opportunities in many biomedical sensing and diagnostic applications where specific binding and release of target analytes are desired.