Selective and Reversible Binding of Thiol-Functionalized Biomolecules on Polymers Prepared via Chemical Vapor Deposition Polymerization


Ross A., Durmaz H. , CHENG K., DENG X., Liu Y., Oh J., ...More

LANGMUIR, vol.31, no.18, pp.5123-5129, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 18
  • Publication Date: 2015
  • Doi Number: 10.1021/acs.langmuir.5b00654
  • Title of Journal : LANGMUIR
  • Page Numbers: pp.5123-5129

Abstract

We use chemical vapor deposition polymerization to prepare a novel dibromomaleimide-functionalized polymer for selective and reversible binding of thiol-containing biomolecules on a broad range of Substrates. We report the synthesis and CVD polymerization of 4-(3,4-dibromomaleimide)[2.2]paracyclophane to yield nanometer thick polymer coatings. Fourier transformed infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the chemical composition of the polymer coating. The reactivity of the polymer coating toward thiol-functionalized molecules was confirmed using fluorescent ligands. As a proof of concept, the binding and subsequent release of cysteine-modified peptides from the polymer coating were also demonstrated via sum frequency generation spectroscopy. This reactive polymer coating provides a flexible surface modification approach to selectively and reversibly bind biomolecules on a broad range of materials, which Could open up new opportunities in many biomedical sensing and diagnostic applications where specific binding and release of target analytes are desired.