Separation and Sensitive Detection of Lanthanides by Capillary Electrophoresis and Contactless Conductivity Detection


YELKENCI H. E. , Öztekin N.

JOURNAL OF CHROMATOGRAPHIC SCIENCE, cilt.55, ss.465-470, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 55 Konu: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1093/chromsci/bmw200
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHIC SCIENCE
  • Sayfa Sayıları: ss.465-470

Özet

A rapid and sensitive capillary zone electrophoresis method for the simultaneous separation and determination of lanthanide ions, combined with capacitively coupled contactless conductivity detection ((CD)-D-4), is reported. The influence of experimental parameters on separation was investigated. The optimal separation conditions were obtained when 4.5 mM 2-hydroxyisobutyric acid and 1 mM acetic acid (HAc) at pH 4.5 were used as buffer solution. Under these conditions, complete separation of all 14 lanthanide ions was achieved in 6 min. With the use of the (CD)-D-4 detector, the sensitive detection of non-UV active lanthanide ions was achieved without the need of a UV active ligand or a visualization agent. The sensitivities were further enhanced with a sample stacking procedure. The limits of detection were to be found between 2.77 and 8.26 nmol/L and the limits of quantification were between 9.29 and 27.5 nmol/L for 14 lanthanide ions.