Cytotoxic effect of extract from Dunaliella salina against SH-SY5Y neuroblastoma cells

Ataseyer-Arslan B., Yilancioglu K., Bekaroglu M. G. , TAŞKIN E., ALTINÖZ E., Cetiner S.

GENERAL PHYSIOLOGY AND BIOPHYSICS, vol.34, no.2, pp.201-207, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.4149/gpb_2014034
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.201-207
  • Istanbul Technical University Affiliated: No


Cytotoxic effects of essential oils extracted from Dunaliella sauna on SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determination of the composition of essential oils found in Dunaliella salina extract. All experimented concentrations of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than the tested concentrations of the extract on ECV304 human endothelial cells used as a control. Fifthy compounds were detected in GC-MS analysis of the extract, and five major compounds were predominantly found as follows: octadecanoic acid, methyl ester (27.43%); hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39%); octadecanoic acid (5.03%), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells might be due to high concentrations of octadecanoic acid and hexadecanoic acid. Furthermore, results indicate that the extract demonstrates some proliferative effect on ECV304 cells in a dose-dependent manner between 0.25 and 5 mu g/ml. These results suggest that Dunaliella sauna may have anticancer potential against human neuroblastoma cells.