In this study, the effect of activation time and flow rate of CO2 on the SO2 adsorption properties of activated carbon nanofiber has been investigated. The activation process was performed under different CO2 flow rates (100 and 150 mL/min) and activation times (30 and 60 minutes). At the FTIR spectra, the formation of a new peak at around 1050 cm(-1) after SO2 adsorption test, showed the presence of SO2 adsorbed on the activated carbon nanofibers. The elemental analyses also confirmed the presence of S atom in the analysed sample after SO2 adsorption test. It has been seen that an increase in activation time and decrease in CO2 feeding rate resulted in an increase in SO2 adsorption capability of activated carbon nanofibers. Rather than the flow rate of the activation gas, activation time has higher effect on the SO2 adsorption capability of activated carbon nanofibers.