The uniform microporous organic polymer (MOP) was obtained in one-pot and one-step polymerization process through Schiff base chemistry under thermal conditions. The obtained anthraquinone-melamine based MOP (AM-MOP) displays high surface area (215m(2)/g). AM-MOP was manually filled into a column with high pressure and used as packing material in the high-performance liquid chromatographic applications. The analysis of compounds such as polycyclic aromatic hydrocarbons, pesticides, flavonoids, and phenolic acids was performed to display a possible application by using AM-MOP packed column. Compared to the commercial columns, AM-MOP exhibits better selectivity factor and column efficiency arising from its strong pi-pi interaction activity. Furthermore, it has similar solvent uptake in both polar and nonpolar media, which explains good compatibility of the material with all mobile phases, from acetonitrile to methanol and water.