© 2014 Bentham Science Publishers.The present study contain the synthesis of enoxacin (ENO) complexes with transition metals of Cu(II), Zn(II), Pt(II), Fe(III) and Ru(III). The structure of the complexes was verified on the basis of elemental analyses, IR, UV-Vis, NMR, mass, electrochemical and thermal spectral data .The electrochemical properties of all compounds were studied by cyclic voltammetry (CV) using a glassy carbon electrode (GCE). The interaction of metal complexes with calf thymus DNA (CT-DNA) was studied with UV spectroscopy. UV studies of the interaction of the compounds with DNA reveal that these complexes can bind to CT-DNA and the binding constants have been calculated. The antimicrobial and antifungal activity of the complexes have been studied against Gram-positive and Gram-negative microorganisms and fungi’s and compared with the reference drug ENO. However, the zinc complex was found to be more potent versus four bacterial species than the bare drug ENO.