A New Approach for in vitro Imaging of Breast Cancer Cells by Anti-Metadherin Targeted PVA-Pyrene

MEDİNE E. İ., Odaci D., GACAL B. N., GACAL B., Sakarya S., ÜNAK P., ...Daha Fazla

MACROMOLECULAR BIOSCIENCE, cilt.10, sa.6, ss.657-663, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 6
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1002/mabi.201000009
  • Dergi Adı: MACROMOLECULAR BIOSCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.657-663
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous