Nanostructured Amphiphilic Star-Hyperbranched Block Copolymers for Drug Delivery


Seleci M., Seleci D. A. , CIFTCI M. , Demirkol D. O. , STAHL F., TİMUR S., ...Daha Fazla

LANGMUIR, cilt.31, ss.4542-4551, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 31 Konu: 15
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/acs.langmuir.5b00082
  • Dergi Adı: LANGMUIR
  • Sayfa Sayıları: ss.4542-4551

Özet

A robust drug delivery system based on nanosized amphiphilic star-hyperbranched block copolymer, namely, poly(methyl methacrylate-block-poly(hydroxylethyl methacrylate) (PMMA-b-PHEMA) is described. PMMA-b-PHEMA was prepared by sequential visible light induced self-condensing vinyl polymerization (SCVP) and conventional vinyl polymerization. All of the synthesis and characterization details of the conjugates are reported. To accomplish tumor cell targeting property, initially cell-targeting (arginylglycylaspactic acid; RGD) and penetrating peptides (Cys-TAT) were binding to each other via the well-known EDC/NHS chemistry. Then, the resulting peptide was further incorporated to the surface of the amphiphilic hyperbranched copolymer via a coupling reaction between the thiol (-SH) group of the peptide and the hydroxyl group of copolymer by using N-(p-maleinimidophenyl) isocyanate as a heterolinker. The drug release property and targeting effect of the anticancer drug (doxorobucin; DOX) loaded nanostructures to two different cell lines were evaluated in vitro. U87 and MCF-7 were chosen as integrin alpha(v)beta(3) receptor positive and negative cells for the comparison of the targeting efficiency, respectively. The data showed that drug-loaded copolymers exhibited enhanced cell inhibition toward U87 cells in compared to MCF-7 cells because targeting increased the cytotoxicity of drug-loaded copolymers against integrin alpha(v)beta(3) receptor expressing tumor cells.