Effects of disulphide bridges on the activity and stability of the formate dehydrogenase from Candida methylica

Karagueler N. G., Sessions R. B., Clarke A. R.

BIOTECHNOLOGY LETTERS, cilt.29, sa.9, ss.1375-1380, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 9
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s10529-007-9392-8
  • Dergi Adı: BIOTECHNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1375-1380
  • İstanbul Teknik Üniversitesi Adresli: Hayır

Özet

Wild-type cmFDH contains no cystines, hence it is a good candidate to test the hypothesis that thermostability can be achieved by introducing new disulphide bridges. Three cysteine double mutants of cmFDH were designed, using a homology model reported previously, to introduce cystine bridges in the C-domain (T169C-T226C) in the N-domain (V88C-V112C) and between the two monomers (M156C-L159C) to form two cystine bridges across the dimer interface. These mutants were constructed and the proteins were over-expressed in E. coli. The mutants V88C-V112C and M156C-L159C lost FDH activity. The mutant T169C-T226C was both less active and less thermostable than wild-type FDH.