Darlik Dam supplies 15% of the water demand of Istanbul Metropolitan City of Turkey. Water quality (WQ) in the Darlik Dam was investigated from Landsat 5 TM satellite images of the years 2004, 2005, and 2006 in order to determine land use/land cover changes in the watershed of the dam that may deteriorate its WQ. The images were geometrically and atmospherically corrected for WQ analysis. Next, an investigation was made by multiple regression analysis between the unitless planetary reflectance values of the first four bands of the June 2005 Landsat TM image of the dam and WQ parameters, such as chlorophyll-a, total dissolved matter, turbidity, total phosphorous, and total nitrogen, measured at satellite image acquisition time at seven stations in the dam. Finally, WQ in the dam was studied from satellite images of the years 2004, 2005, and 2006 by pattern recognition techniques in order to determine possible water pollution in the dam. This study was compared to a previous study done by the authors in the Kucukcekmece water reservoir, also in Istanbul City.