Microseconds Simulations Reveal a New Sodium-binding Site and the Mechanism of Sodium-coupled Substrate Uptake by LeuT

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Zomot E., Gur M., BAHAR I.

JOURNAL OF BIOLOGICAL CHEMISTRY, vol.290, no.1, pp.544-555, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 290 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1074/jbc.m114.617555
  • Journal Name: JOURNAL OF BIOLOGICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.544-555
  • Istanbul Technical University Affiliated: Yes

Abstract

Background: LeuT is a homologue of neurotransmitter transporters. Results: Microseconds simulations disclose multiple translocation sites for Na+, including a new site for initial binding. Conclusion: Coupled Na+- and substrate-binding events are accompanied by local and global (interhelical) rearrangements in the outward facing structure. Significance: New insights are gained into Na+ and substrate uptake/efflux mechanisms of LeuT.