Microseconds Simulations Reveal a New Sodium-binding Site and the Mechanism of Sodium-coupled Substrate Uptake by LeuT

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Zomot E., Gur M., BAHAR I.

JOURNAL OF BIOLOGICAL CHEMISTRY, cilt.290, sa.1, ss.544-555, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 290 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1074/jbc.m114.617555
  • Dergi Adı: JOURNAL OF BIOLOGICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.544-555
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Background: LeuT is a homologue of neurotransmitter transporters. Results: Microseconds simulations disclose multiple translocation sites for Na+, including a new site for initial binding. Conclusion: Coupled Na+- and substrate-binding events are accompanied by local and global (interhelical) rearrangements in the outward facing structure. Significance: New insights are gained into Na+ and substrate uptake/efflux mechanisms of LeuT.