Evolutionary engineering and transcriptomic analysis of nickel-resistant Saccharomyces cerevisiae


Creative Commons License

Kucukgoze G., ALKIM C., YILMAZ U., KISAKESEN H. I. , GUNDUZ S., AKMAN S. , ...Daha Fazla

FEMS YEAST RESEARCH, cilt.13, ss.731-746, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 13 Konu: 8
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1111/1567-1364.12073
  • Dergi Adı: FEMS YEAST RESEARCH
  • Sayfa Sayıları: ss.731-746

Özet

Increased exposure to nickel compounds and alloys due to industrial development has resulted in nickel pollution and many pathological effects on human health. However, there is very limited information about nickel response, transport, and tolerance in eukaryotes. To investigate nickel resistance in the model eukaryote Saccharomyces cerevisiae, evolutionary engineering by batch selection under gradually increasing nickel stress levels was performed. Nickel hyper-resistant mutants that could resist up to 5.3mM NiCl2, a lethal level for the reference strain, were selected. The mutants were also cross-resistant against iron, cobalt, zinc, and manganese stresses and accumulated more than twofold higher nickel than the reference strain. Global transcriptomic analysis revealed that 640 upregulated genes were related to iron homeostasis, stress response, and oxidative damage, implying that nickel resistance may share common mechanisms with iron and cobalt resistance, general stress response, and oxidative damage.