Evolutionary engineering and transcriptomic analysis of nickel-resistant Saccharomyces cerevisiae

Creative Commons License

Kucukgoze G., ALKIM C., YILMAZ U., KISAKESEN H. I., GUNDUZ S., AKMAN S., ...More

FEMS YEAST RESEARCH, vol.13, no.8, pp.731-746, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 8
  • Publication Date: 2013
  • Doi Number: 10.1111/1567-1364.12073
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.731-746
  • Keywords: evolutionary engineering, inverse metabolic engineering, yeast DNA microarray analysis, nickel resistance, Saccharomyces cerevisiae, stress resistance, IRON REGULON, FET3 GENE, YEAST, TOLERANCE, TRANSPORT, STRESS, COBALT, METABOLISM, ACTIVATION, MECHANISMS
  • Istanbul Technical University Affiliated: Yes


Increased exposure to nickel compounds and alloys due to industrial development has resulted in nickel pollution and many pathological effects on human health. However, there is very limited information about nickel response, transport, and tolerance in eukaryotes. To investigate nickel resistance in the model eukaryote Saccharomyces cerevisiae, evolutionary engineering by batch selection under gradually increasing nickel stress levels was performed. Nickel hyper-resistant mutants that could resist up to 5.3mM NiCl2, a lethal level for the reference strain, were selected. The mutants were also cross-resistant against iron, cobalt, zinc, and manganese stresses and accumulated more than twofold higher nickel than the reference strain. Global transcriptomic analysis revealed that 640 upregulated genes were related to iron homeostasis, stress response, and oxidative damage, implying that nickel resistance may share common mechanisms with iron and cobalt resistance, general stress response, and oxidative damage.