Enzymatic esterification of (-)-menthol with fatty acids in solvent by a commercial lipase from Candida rugosa

Babali B., AKSOY H., Tuter M., USTUN G.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, vol.78, no.1, pp.53-56, 2001 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 1
  • Publication Date: 2001
  • Doi Number: 10.1007/s11746-001-0219-x
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.53-56
  • Keywords: Candida cylindracea lipase, Candida rugosa lipase, enzymatic esterification, fatty acid, (-)-menthol, IMMOBILIZED LIPASE, ORGANIC-SOLVENTS, DL-MENTHOL, MICROEMULSIONS, SELECTIVITY, MEDIA, OIL
  • Istanbul Technical University Affiliated: No


Esterification of (-)-menthol with fatty acids in isooctane was successfully catalyzed using a commercial lipase, Lipase AY ''Amano" 30 from Candida rugosa in original powder form. The esterification reactions were performed to elucidate the effects of temperature, enzyme load, molar ratio of (-)-menthol/fatty acid, and fatty acid type, keeping the (-)-menthol concentration at 200 mM. At the optimal conditions for (-)-menthol esterification, determined at a (-)-menthol/lauric acid molar ratio of 1:1 and 35 degreesC [1.5 g enzyme/g (-)-menthol, 0.1 g molecular sieves], the molar conversion of (-)-menthol after 48 h reached 93%. After 24 h, the lowest and the highest molar conversions of fatty acids at 2:1 molar ratio were obtained with myristic acid (71%) and margaric acid (98%), respectively. After 48 h, the molar conversions of lauric acid at molar ratios 2.1, 1:1, and 1:2 were 98, 93, and 49%, respectively.