A colour normalization method for Giemsa-stained blood cell images

Tek F. B., Dempster A. G., Kale I.

IEEE 14th Signal Processing and Communications Applications, Antalya, Turkey, 16 - 19 April 2006, pp.559-560 identifier identifier

  • Publication Type: Conference Paper / Full Text
  • Doi Number: 10.1109/siu.2006.1659768
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.559-560
  • Istanbul Technical University Affiliated: No


This paper presents a novel method for the colour normalization of Giemsa-stained peripheral blood cell images. The normalization is applied separately to the foreground and background regions. A rough estimation of the foreground-background regions is done by mathematical morphology and followed by a refined segmentation using histograms of these regions. Then an illumination independent response is calculated using the background region. The normalization is completed by transforming the foreground region according to a reference set. The proposed method has been tested on many images and has been found successful.