Crosslinking of electrospun and bioextruded partially hydrolyzed poly(2-ethyl-2-oxazoline) using glutaraldehyde vapour

Kalaoglu-Altan Ö. İ., Li Y., McMaster R., Shaw A., Hou Z., Vergaelen M., ...More

European Polymer Journal, vol.120, 2019 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 120
  • Publication Date: 2019
  • Doi Number: 10.1016/j.eurpolymj.2019.109218
  • Journal Name: European Polymer Journal
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Bioextrusion, Crosslinking, Electrospinning, Fused deposition modelling, Glutaraldehyde, Poly(2-ethyl-2-oxazoline)
  • Istanbul Technical University Affiliated: No


Poly(2-ethyl-2-oxazoline)s (PEtOx) have received substantial attention for various potential biomedical applications, yet they have not been explored as scaffold materials to any extensive degree. A major challenge to open up future applications is to overcome the poor water stability of these materials. We here propose a universal crosslinking strategy for these materials based on a partial acidic hydrolysis of PEtOx to poly[(2-ethyl-2-oxazoline)-co-(ethylenimine)] (PEtOx-EI) followed by exposure to glutaraldehyde vapour to create water-stable scaffolds. To demonstrate the utility of this approach two different fabrication techniques were used to make 2- and 3-dimensional structures, namely solution electrospinning and fused deposition modelling (FDM). Because the partial hydrolysis results in increased hydrophilicity, the crosslinking conditions for the fine PEtOx-EI nanofibers were carefully tuned to enable crosslinking of the nanofibers prior to a loss of the nanofibrous morphology. Conversely, for the thicker FDM printed PEtOx-EI structures the crosslinking conditions are more tolerant. Crosslinking with glutaraldehyde vapour provided water-stability to both 2D and 3D constructs, which is an important asset for biomedical applications.