Polyethyleneimine-coated capillaries for the separation of DNA by capillary electrophoresis


Nutku M., Berker F. B.

TURKISH JOURNAL OF CHEMISTRY, vol.27, no.1, pp.9-14, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 1
  • Publication Date: 2003
  • Journal Name: TURKISH JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.9-14
  • Keywords: capillary electrophoresis, DNA, polyethyleneimine, MOLECULAR-MASS POLYETHYLENEIMINE, ENTANGLED POLYMER-SOLUTIONS, RESTRICTION FRAGMENTS, ZONE ELECTROPHORESIS, CATIONIC POLYMER, PERFORMANCE, OLIGONUCLEOTIDES, PROTEINS, PEPTIDES
  • Istanbul Technical University Affiliated: Yes

Abstract

A new capillary electrophoresis of DNA fragments has been achieved with the cooperative effects of the electrophoretic and electroosmotic mobilities realized via the polyethyleneimine (PEI) coating of the capillary walls. As a result, seven of the 11 different-sized DNA fragments have produced resolved CE peaks. Our results demonstrate that analyses of DNA constituents can be performed under a wide range of conditions by the appropriate tuning of the coating and filler within the capillary.