A Preliminary Study on Cell Motility Analysis from Phase-Contrast Microscopy Image Series Faz-Kontrast Mikroskopi Goruntu Serilerinden Hucre Hareketliligi Analizi On-Calismasi

Kayan E., Kavusan T., Önal S., Okvur D. P., Özuysal Ö. Y., Töreyin B. U., ...Daha Fazla

2020 Medical Technologies Congress, TIPTEKNO 2020, Antalya, Türkiye, 19 - 20 Kasım 2020 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası:
  • Doi Numarası: 10.1109/tiptekno50054.2020.9299319
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

© 2020 IEEE.Analyses of morphology, polarity, and motility of cells is important for cell biology research such as metastatic and invasive capacity of cells, wound healing, and embryonic development. Automation of such analyses using image series of phase-contrast optical microscopy, which allows label-free imaging of live cells in their living environment, is a need. With this purpose, in this study image series of a cell motility experiment is manually annotated, and an automation algorithm realizing motion and shape analyses of cells using the annotated data is developed. In addition, due to the low number of annotated data at hand, a U-Net based solution is devised for automated segmentation of the cells and its performance is evaluated.