Capillary electrokinetic separation of polycyclic aromatic hydrocarbons using cetylpyridinium bromide


BELIN G., Erim F. B. , GULACAR F.

POLYCYCLIC AROMATIC COMPOUNDS, vol.24, pp.343-352, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 24
  • Publication Date: 2004
  • Doi Number: 10.1080/10406630490468504
  • Title of Journal : POLYCYCLIC AROMATIC COMPOUNDS
  • Page Numbers: pp.343-352

Abstract

Polycyclic aromatic hydrocarbons (PAHs) including isomeric pairs were separated in capillary electrokinetic chromatography using a cationic surfactant cetylpyridinium bromide (CPBr) as additive. With addition of 2 mM CPBr into the running electrolyte, dynamic coating occurs in the capillary and EOF is reversed. Changes of electroosmotic and electrophoretic mobilities with increasing CPBr concentration were investigated. Under optimum separation conditions, running electrolyte contains 50% MeCN, 20 mM acetate, and 40 mM CPBr at pH = 4.0. Using high concentration of organic solvent, aggregation of surfactants into micelles is prevented. Significant retentions indicate solvophobic, n- and pi-electron interactions between CPBr monomers and PAHs.