The aim of this study was to evaluate the effects of LPUVC253.7 nm and LEDs405.5 nm light on sweet and sour cherries, to assess the potential of post-harvest illumination for fruit shelf-life and quality extension. Fresh cherries were treated at several doses (up to 1.56 kJ m−2) in a lab design chamber. Microbiological parameters, total phenolic content, antioxidant activity, and quality properties such as weight loss, color, pH, and total soluble solids content, were assessed before and after illumination and during storage time up to 24 days at 4 and 25 °C. Studies on inactivation of natural fruit microbiota and artificially inoculated potential spoilage bacteria Pseudomonas syringae and Aureobasidium pullulans were performed. A maximum reduction of 1.89 and 1.66 log on total aerobic mesophilic bacteria and total mold and yeasts was obtained after light illumination at 253.7 nm on spiked cherry fruits. Regarding fruit quality properties, both light (LPUVC253.7 nm and LEDs405.5 nm) illumination caused no change (p > 0.05) in quality parameters compared with non-treated fruits. Industrial relevance: There is an increasing global demand from consumers for agricultural products high nutritional quality with no or minimal chemical preservatives. Postharvest physical ultraviolet-C (UVC) treatment before or during cold storage can inactivate microbial contaminants, alleviate low-temperature damage, reduce postharvest decay, and extend shelf life of fruits while improving chemical safety. The present study indicated that post-harvest LPUVC and LEDs illumination of cherries can be used as a clean and environmental forthcoming process to extend the shelf-life of fruits with highly preserved quality.