Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility


Dincturk H., Dunn A., Jones D.

JOURNAL OF BIOSCIENCES, vol.26, no.5, pp.635-640, 2001 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 5
  • Publication Date: 2001
  • Doi Number: 10.1007/bf02704761
  • Title of Journal : JOURNAL OF BIOSCIENCES
  • Page Numbers: pp.635-640

Abstract

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.