Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility


Dincturk H., Dunn A., Jones D.

JOURNAL OF BIOSCIENCES, cilt.26, ss.635-640, 2001 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 26 Konu: 5
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1007/bf02704761
  • Dergi Adı: JOURNAL OF BIOSCIENCES
  • Sayfa Sayıları: ss.635-640

Özet

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.