Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to antibiofouling strategies in a membrane bioreactor (MBR) for wastewater treatment. However, the target signaling molecules have been limited to N-acyl homoserine lactones participating in intraspecies quorum sensing. Here, an approach to disrupting autoinducer-2 (AI-2) signaling molecules participating in interspecies quorum sensing was pursued as a next-generation antibiofouling strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium (Acinetobacter sp. DKY-1) that can attenuate the expression of the quorumsensing (QS) response through the inactivation of an autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD), among four kinds of autoinducer-2 QS bacteria. hydrophilic with a molecular weight of <400 Da. The addition DPD concentration and remarkably reduced membrane bio wastewater engineering, could enlarge the range of QQ-MBR treatment. DKY-1 released AI-2 QQ compounds, which were verified to be of DKY-1 entrapping beads into an MBR significantly decreased fouling. This new approach, combining molecular biology with for antibiofouling and energy savings in the field of wastewater treatment.