Stopping Autoinducer-2 Chatter by Means of an Indigenous Bacterium (Acinetobacter sp DKY-1): A New Antibiofouling Strategy in a Membrane Bioreactor for Wastewater Treatment


Lee K., Kim Y., Lee S., Lee S. H. , Nahm C. H. , Kwon H., ...More

ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol.52, no.11, pp.6237-6245, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 11
  • Publication Date: 2018
  • Doi Number: 10.1021/acs.est.7b05824
  • Journal Name: ENVIRONMENTAL SCIENCE & TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6237-6245
  • Istanbul Technical University Affiliated: Yes

Abstract

Bacterial quorum quenching (QQ) by means of degrading signaling molecules has been applied to antibiofouling strategies in a membrane bioreactor (MBR) for wastewater treatment. However, the target signaling molecules have been limited to N-acyl homoserine lactones participating in intraspecies quorum sensing. Here, an approach to disrupting autoinducer-2 (AI-2) signaling molecules participating in interspecies quorum sensing was pursued as a next-generation antibiofouling strategy in an MBR for wastewater treatment. We isolated an indigenous QQ bacterium (Acinetobacter sp. DKY-1) that can attenuate the expression of the quorumsensing (QS) response through the inactivation of an autoinducer-2 signaling molecule, 4,5-dihydroxy-2,3-pentanedione (DPD), among four kinds of autoinducer-2 QS bacteria. hydrophilic with a molecular weight of <400 Da. The addition DPD concentration and remarkably reduced membrane bio wastewater engineering, could enlarge the range of QQ-MBR treatment. DKY-1 released AI-2 QQ compounds, which were verified to be of DKY-1 entrapping beads into an MBR significantly decreased fouling. This new approach, combining molecular biology with for antibiofouling and energy savings in the field of wastewater treatment.