A Molecularly Imprinted Polymer Based Biosensor for Electrochemical Impedance Spectroscopic Analysis

UTKU F. S. , Ozdemir O. E. , Bakay M. S.

ELECTRICA, cilt.18, ss.39-44, 2018 (ESCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 18 Konu: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.5152/iujeee.2018.1807
  • Dergi Adı: ELECTRICA
  • Sayfa Sayıları: ss.39-44


A molecularly imprinted polymer (MIP)-based impedimetric biosensor was developed for the electrochemical analysis of low-weight biological molecules. Synthetic polymeric matrices with specific and selective recognition sites, which are complementary to the shapes and sizes of the functional groups of a na lytes, can be prepared using the molecular imprinting method. In this study, a small molecule, tris(hydroxymethyl)aminomethane (IRIS), was used to coat a graphite pencil tip with a TRIS-containing polyacrylamide gel to fabricate a working electrode.The electrode modification and performance were evaluated using cyclic voltammetry and electrochemical impedance spectroscopy.The electrochemical properties of the modified electrodes were observed using an electrochemical cell comprising a Ag/AgCl reference electrode, a Pt wire as the counter electrode, and a pencil graphite tip as the working electrode using a redox-phosphate buffer solution with different concentrations of TRIS and Ethylenediaminetetraacetic acid (EDTA).The I-V and impedance performance of the chemically modified graphite pencil-tip electrodes exhibited decreased conductance and increased impedance correlating with the increase in IRIS concentration.Thus, MIP-based small-molecule biosensor prototypes can be promising economical replacements over other expensive sensors.