Characteristics of mixed microbial culture at different sludge ages: Effect on variable kinetics for substrate utilization


PALA-OZKOK I., REHMAN A., Yağcı N., Ubay-Cokgor E., JONAS D., Orhon D.

BIORESOURCE TECHNOLOGY, cilt.126, ss.274-282, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 126
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.biortech.2012.08.115
  • Dergi Adı: BIORESOURCE TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.274-282
  • Anahtar Kelimeler: Modeling, Respirometry, Intracellular storage, Microbial population, Pyrosequencing, WATER TREATMENT-PLANT, ACTIVATED-SLUDGE, WASTE-WATER, RESPIROMETRIC EVALUATION, BACTERIAL COMMUNITY, MEMBRANE BIOREACTOR, GROWTH-KINETICS, REACTOR, BIODEGRADATION, NITRIFICATION
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

The study focused on variable kinetics for substrate utilization, primarily addressing the following issue: Is variable process kinetics observed under different operating conditions and culture history (sludge ages), the result of changes inflicted on the metabolic machinery of the same microbial culture? Or, is this the result of a different microbial population selected under different operating conditions? For this purpose, the study mainly emphasized to assess the microbial population composition sustained at different sludge ages. It explored the relationship between observed process kinetics and microbial population structure using respirometric modeling and high-throughput sequencing of 16S rRNA gene. Experimental results indicated a significant change in the composition of the microbial community fed with the same organic substrate, when the culture history was changed, lower sludge age selecting a different and faster growing microbial community. (C) 2012 Elsevier Ltd. All rights reserved.