Glycidyl methacrylate grafted on p(VBC) beads by SI-ATRP technique: Modified with hydrazine as a salt resistance ligand for adsorption of invertase


Bayramoglu G., YAVUZ E. , Şenkal B. F. , Arica M. Y.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, cilt.345, ss.127-134, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 345
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.colsurfa.2009.04.044
  • Dergi Adı: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
  • Sayfa Sayıları: ss.127-134

Özet

The cross-linked p(vinyl benzyl chloride), p(VBC), beads were prepared by suspension polymerization, and p(glycidylmethacrylate), p(GMA), was grafted on the surface of the beads using surface-initiated atom transfer radical polymerization, (SI-ATRP). The epoxy groups of the p(GMA) were reacted with a salt resistance ligand (i.e., hydrazine). The hydrazine-functionalized beads were used for adsorption of invertase. The influence of pH, equilibrium time, ionic strength, temperature and initial invertase concentration on the adsorption capacity of the affinity beads has been investigated. Maximum invertase adsorption onto affinity beads was 86.7 mg/g at pH 4.0. The experimental equilibrium data fitted well to the Dubinin-Radushkevich isotherm models. (C) 2009 Elsevier B.V. All rights reserved.