Electrochemical deposition of polypeptides: bio-based covering materials for surface design

Akbulut H., Yavuz M., Guler E., ODACI DEMİRKOL D., Endo T., Yamada S., ...Daha Fazla

POLYMER CHEMISTRY, cilt.5, sa.12, ss.3929-3936, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 12
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1039/c4py00079j
  • Dergi Adı: POLYMER CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3929-3936
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

A simple and efficient approach for the electrochemical deposition of polypeptides as bio-based covering materials for surface design is described. The method involves N-carboxyanhydride (NCA) ring-opening polymerization from its precursor to form a thiophene-functionalized polypeptide macromonomer (T-Pala), followed by electropolymerization. The obtained conducting polymer, namely polythiophene-g- polyalanine (PT-Pala), was characterized and utilized as a matrix for biomolecule attachment. The biosensing applicability of PT-Pala was also investigated by using glucose oxidase (GOx) as a model enzyme to detect glucose. The designed biosensor showed a very good linearity for 0.01-1.0 mM glucose. Finally, the antimicrobial activities of newly synthesized T-Pala and PT-Pala were also evaluated by using the disc diffusion method.