APPLICATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY AND INDIRECT UV DETECTION FOR THE ANALYSIS OF FATTY-ACIDS

ERIM F. B., Xu X., Kraak J.

JOURNAL OF CHROMATOGRAPHY A, vol.694, no.2, pp.471-479, 1995 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 694 Issue: 2
  • Publication Date: 1995
  • Doi Number: 10.1016/0021-9673(94)01114-t
  • Journal Name: JOURNAL OF CHROMATOGRAPHY A
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.471-479
  • Istanbul Technical University Affiliated: Yes

Abstract

A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C-8-C-20 fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.