APPLICATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY AND INDIRECT UV DETECTION FOR THE ANALYSIS OF FATTY-ACIDS


ERIM F. B. , Xu X., Kraak J.

JOURNAL OF CHROMATOGRAPHY A, cilt.694, ss.471-479, 1995 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 694 Konu: 2
  • Basım Tarihi: 1995
  • Doi Numarası: 10.1016/0021-9673(94)01114-t
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Sayfa Sayıları: ss.471-479

Özet

A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C-8-C-20 fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.