In this study, Ecballium elaterium and P. mahaleb seed oils were used in the preparation of urethane oil. It is well known that urethane oils are prepared by reacting a diisocyanate with the partial esters obtained from triglyceride oil. Consequently, urethane oils can be considered as an alkyd resin in which the phthalic anhydride is replaced by a diisocyanate. Film properties of these materials are dependent on the oil, polyol, and diisocyanate used in the formulation. Since Ecballium elaterium and P. mahaleb seed oils contain punicic and alpha-eleostearic acids, respectively, which are conjugated trienoic acids, the use of these oils as an oil component in the urethane oils seems to be worth investigating. Although these oils contain conjugated double bonds, no gelation occurred during the process. In addition to these oils, dehydrated castor oil and linseed oil were also included in the study to obtain comparative samples. An investigation of the film properties indicated that Ecballium elaterium and P. mahaleb seed oils could be used as ingredients of urethane oils.