The rate at which particles deposit and re-suspend from indoor surfaces is an essential parameter in determining human exposure to aerosol particles. In this study, we investigate experimentally particle deposition and forced re-suspension in a cubical enclosure for three particle sizes (0.7, 1.5 and 4 mu m). For particle deposition, the results are presented in terms of particle deposition velocity. It was observed that the particle deposition velocity is particularly significant for the floor surface. Deposition on vertical and ceiling surfaces becomes important only when these surfaces are electrically charged. Air humidity did not influence the particle deposition apart from when it was close to saturation. Re-suspension of particles from the floor surface due to household activities was also studied. It was observed that small particles are more difficult to detach from the floor than bigger ones. Besides, there is a pressure above which re-suspension is very likely to occur (minimum re-suspension pressure). This minimum pressure is dependent on the particle size.