A novel determination method for diuron in seaweed samples: Combination of quadruple isotope dilution strategy with liquid chromatography - quadrupole time of flight - tandem mass spectrometry for superior accuracy and precision


Tekin Z., ÖZTÜRK ER E., GÜNKARA Ö. T., BAKIRDERE S.

JOURNAL OF CHROMATOGRAPHY A, vol.1611, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1611
  • Publication Date: 2020
  • Doi Number: 10.1016/j.chroma.2019.460612
  • Journal Name: JOURNAL OF CHROMATOGRAPHY A
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Keywords: Diuron, Isotope dilution, Seaweed, Mass spectrometry, SURFACE-WATER, HERBICIDES, EXTRACTION, PESTICIDES, MS/MS, ESI, MS
  • Istanbul Technical University Affiliated: No

Abstract

This paper proposed an accurate and sensitive analytical method based on the combination of Liquid Chromatography-Quadrupole Time of Flight-Tandem Mass Spectrometry (LC-QTOF-MS/MS) system with Quadruple Isotope Dilution-Mass Spectrometry ((IDMS)-M-4) strategy for the determination of diuron at trace levels. In order to achieve accurate and reliable determination of the analyte, (IDMS)-M-4 strategy that uses stable isotopically labelled analogues was employed. Diuron-d6 was synthesized in the laboratory using a novel strategy and employed in (IDMS)-M-4 for the preparation of three calibration blends and seaweed sample blend. The analytical performance of the LC-QTOF-MS/MS method was evaluated and the respective detection and quantification limits obtained were 14.6 mu g kg(-1) and 46.5 mu g kg(-1). Good linearity was obtained with a correlation coefficient of 0.9995 over the dynamic range from 50 to 1000 mu g kg(-1). The validity of the method was successfully tested by carrying out spiking experiments in seaweed samples. The percent recovery value showed superior enhancement for (IDMS)-M-4 strategy (99.97 +/- 0.41%) confirming the applicability of the method to complex matrices with great accuracy and precision. (C) 2019 Elsevier B.V. All rights reserved.