One of the negative effects of transport on environment is the land use. The land which is suitable for agriculture is being destroyed during the construction of transport systems, considerable quantities of green areas are being destroyed and the ecological balance is being damaged due to transport systems. In the Studies conducted, the areas required by the transport systems are determined and the costs of these areas are calculated. Such a study is not conducted for Istanbul, which is one of the most important cities of Turkey, with a population of 12 millions. In the study that is conducted in order to fill this gap and by using the number of passengers transported and the area occupied by two land transport systems for Istanbul city, which are highways and railroads, the areas per unit and the ecological factors for these areas are examined.