Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions


Akduman I. , BRAND U., GROCHMALICKI J., HESTER G., PIKE R., BERTERO M.

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, cilt.15, ss.2275-2287, 1998 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 15 Konu: 9
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1364/josaa.15.002275
  • Dergi Adı: JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
  • Sayfa Sayıları: ss.2275-2287

Özet

A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA = 1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated. (C) 1998 Optical Society of America.